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pSEVAD1Z1A
(Plasmid #58297)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58297 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli MC1061 DIAL EI ΔdapA-Eps
  • Growth instructions
    For plasmid propagation, a host with an integrated GeneGuard genomic cassette is required.
  • Copy number
    Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DIAL strains. Part source: Prof J. Christopher Anderson (Dept of Bioengineering, University of California, Berkeley);COR parts. Part source: Prof Vićtor de Lorenzo (Systems Biology Program, Centro Nacional de Biotecnologiá, Madrid); Zeta/Epsilon. Toxin-Antitoxin parts. Part source: Dr. Urszula Zielenkiewicz (Dept of Microbial Biochemistry, Institute of Biochemistry and Biophysics of the Polish Academy of Sciences).
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEVAD1Z1A was a gift from Tom Ellis (Addgene plasmid # 58297 ; http://n2t.net/addgene:58297 ; RRID:Addgene_58297)
  • For your References section:

    GeneGuard: a Modular Plasmid System Designed for Biosafety. Wright O, Delmans M, Stan GB, Ellis T. ACS Synth Biol. 2014 May 13. 10.1021/sb500234s PubMed 24847673