Flag_MmPERK_S503_N1114_pBabePuro
(Plasmid #58423)

• Purpose: retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant mouse PERK deleted from amino acids M1 to Y502. The remaining amino acids are S503 to N1114.
• Depositing Lab: David Ron
• Publication: Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;110(12):4628-33. doi: 10.1073/pnas.1217611110. Epub 2013 Mar 4.

Backbone

• Vector backbone: pBabe puro
• Backbone manufacturer: Addgene plasmid # 1764
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl2
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PERK
• Alt name: Eukaryotic translation initiation factor 2-alpha kinase 3
• Alt name: PRKR-like endoplasmic reticulum kinase
• Alt name: Eif2ak3
• Species: M. musculus (mouse)
• Mutation: Deleted amino acids M1 to Y502
• Entrez Gene: Eif2ak3 (a.k.a. Pek, Perk)
• Tags / Fusion Proteins:
  • preprotrypsin signal sequence (N terminal on insert)
  • FLAG

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pLXSN 5
• 3′ sequencing primer: pBABE-3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry