pcDNA3.1-hNoxa1
(Plasmid #58531)

• Purpose: expresses human Noxa1 in mammalian cells
• Depositing Labs: Botond Banfi, Karl-Heinz Krause
• Publication: Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. Epub 2002 Dec 6.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7100
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): JM109
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NADPH oxidase activator 1
• Alt name: Noxa1
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• Entrez Gene: NOXA1 (a.k.a. NY-CO-31, SDCCAG31, p51NOX)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry