CMV-10-3xFLAG-LGP2-K651E
(Plasmid
#58685)
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PurposeExpresses LGP2 with a mutation in the CTD (K651E) in mammalian cells
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Depositing Lab
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Depositing OrganizationNorthwestern University
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58685 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 58685-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 58685-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonep3X-FLAG-CMV-10
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Backbone manufacturerSigma Aldrich
- Backbone size w/o insert (bp) 6300
- Total vector size (bp) 8400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLGP2-K651E
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Alt nameDHX58
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Alt nameLGP2
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SpeciesH. sapiens (human)
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Insert Size (bp)2037
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Mutationmutation to CTD, K651E
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GenBank IDNM_024119
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Entrez GeneDHX58 (a.k.a. D11LGP2, D11lgp2e, LGP2, RLR-3)
- Promoter CMV
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Tag
/ Fusion Protein
- 3x FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer CMV-R
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byCloned in Horvath Lab by Akihiko Komuro, mutation by Annie Bruns
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 58685-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 58685-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-10-3xFLAG-LGP2-K651E was a gift from Curt Horvath (Addgene plasmid # 58685 ; http://n2t.net/addgene:58685 ; RRID:Addgene_58685) -
For your References section:
ATP hydrolysis enhances RNA recognition and antiviral signal transduction by the innate immune sensor, laboratory of genetics and physiology 2 (LGP2). Bruns AM, Pollpeter D, Hadizadeh N, Myong S, Marko JF, Horvath CM. J Biol Chem. 2013 Jan 11;288(2):938-46. doi: 10.1074/jbc.M112.424416. Epub 2012 Nov 26. 10.1074/jbc.M112.424416 PubMed 23184951