CMV-10-3xFLAG-LGP2-K651E
(Plasmid #58685)

• Purpose: Expresses LGP2 with a mutation in the CTD (K651E) in mammalian cells
• Depositing Lab: Curt Horvath
• Publication: Bruns et al J Biol Chem. 2013 Jan 11;288(2):938-46. doi: 10.1074/jbc.M112.424416. Epub 2012 Nov 26.

Backbone

• Vector backbone: p3X-FLAG-CMV-10
• Backbone manufacturer: Sigma Aldrich
• Backbone size w/o insert (bp): 6300
• Total vector size (bp): 8400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LGP2-K651E
• Alt name: DHX58
• Alt name: LGP2
• Species: H. sapiens (human)
• Insert Size (bp): 2037
• Mutation: mutation to CTD, K651E
• GenBank ID: NM_024119
• Entrez Gene: DHX58 (a.k.a. D11LGP2, D11lgp2e, LGP2, RLR-3)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3x FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: CMV-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cloned in Horvath Lab by Akihiko Komuro, mutation by Annie Bruns

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry