Purposeused for anti-mouse Smoothened antibody production (for injection into animals)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||58702||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesM. musculus (mouse)
Mutationcontains C terminus (aa 543-793)
Entrez GeneSmo (a.k.a. E130215L21Rik, Smoh, bnb, smoothened)
- Promoter tac
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
See antibody production protocol in supplemental documents.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX mSmo-Cterm was a gift from Matthew Scott (Addgene plasmid # 58702 ; http://n2t.net/addgene:58702 ; RRID:Addgene_58702)
For your References section:Patched1 regulates hedgehog signaling at the primary cilium. Rohatgi R, Milenkovic L, Scott MP. Science. 2007 Jul 20;317(5836):372-6. 10.1126/science.1139740 PubMed 17641202