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pX330A_D10A-1x4
(Plasmid #58774)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58774 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    humanized S. pyogenes Cas9 (D10A) nickase
  • Alt name
    SpCas9n (D10A)
  • Alt name
    hSpCas9n (D10A)
  • Species
    S. pyogenes
  • Insert Size (bp)
    4272
  • Mutation
    D10A nickase-converting mutation
  • Promoter CBh
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Feng Zhang
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX330A_D10A-1x4 was a gift from Takashi Yamamoto (Addgene plasmid # 58774 ; http://n2t.net/addgene:58774 ; RRID:Addgene_58774)
  • For your References section:

    Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. 10.1038/srep05400 PubMed 24954249