pCRU5HT1-inLuc
(Plasmid #58954)

• Purpose: HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.
• Depositing Labs: David Derse, Dmitriy Mazurov
• Publication: Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788. doi: 10.1371/journal.ppat.1000788.

Backbone

• Vector backbone: pHTC
• Total vector size (bp): 8970
• Vector type: Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl2
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: inLuc
• Promoter: CMV/R/U5

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Resource Information

• Supplemental Documents:
  • Protocol for generation of HIV-1 and HTLV-1 inLuc vectors with mR-int330 shRNA modification.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC results show a small discrepancy within the intron when compared to the full sequence. This does not have any functional consequence.