pJC30X-BS(X)
(Plasmid
#58958)
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PurposeEncodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.
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Depositing Lab
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Depositing OrganizationCornell University
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 58958 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 58958-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 58958-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBluescript
- Backbone size w/o insert (bp) 2900
- Total vector size (bp) 8300
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEnhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)5400
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MutationGFP modified as described: CORMACK, B. P., R. H. VALDIVIA and S. FALKOW, 1996 FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173: 33-38.
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GenBank IDU89686.1
- Promoter n/a
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Tag
/ Fusion Protein
- First 8 codons of COX3 (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer none
- 3′ sequencing primer none
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 58958-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 58958-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJC30X-BS(X) was a gift from Thomas Fox (Addgene plasmid # 58958 ; http://n2t.net/addgene:58958 ; RRID:Addgene_58958) -
For your References section:
Expression of green fluorescent protein from a recoded gene inserted into Saccharomyces cerevisiae mitochondrial DNA. Cohen JS, Fox TD. Mitochondrion. 2001 Aug;1(2):181-9. S1567-7249(01)00012-5 [pii] PubMed 16120277