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Addgene

pcdna3 Flag Jip2
(Plasmid #59027)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 59027 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcdna3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 7950
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitogen-activated protein kinase 8 interacting protein 2
  • Alt name
    MAPK8IP2
  • Alt name
    Jip2
  • Insert Size (bp)
    2504
  • GenBank ID
    AF136382
  • Entrez Gene
    MAPK8IP2 (a.k.a. IB-2, IB2, JIP2, PRKM8IPL)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcdna3 Flag Jip2 was a gift from Roger Davis (Addgene plasmid # 59027 ; http://n2t.net/addgene:59027 ; RRID:Addgene_59027)
  • For your References section:

    The JIP group of mitogen-activated protein kinase scaffold proteins. Yasuda J, Whitmarsh AJ, Cavanagh J, Sharma M, Davis RJ. Mol Cell Biol. 1999 Oct;19(10):7245-54. PubMed 10490659