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G10 Cas9 MDC123
(Plasmid #59187)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59187 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMDC123
  • Backbone manufacturer
    Mark D Curtis
  • Backbone size w/o insert (bp) 10213
  • Total vector size (bp) 13191
  • Modifications to backbone
    Added G10 promoter instead of the 2X35S promoter and added a Glycine Max Codon Optimized Cas9 with an N and C terminus nuclear localization signal.
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    It may take longer to grow than other plasmids due to the Cas9
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Glycine Max codon optimized Cas9
  • Alt name
    Cas9
  • Species
    Glycine Max
  • Insert Size (bp)
    4140
  • Promoter G10

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer M13_forward20_primer
  • 3′ sequencing primer M13_reverse_primer
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    G10 Cas9 MDC123 was a gift from Robert Stupar (Addgene plasmid # 59187 ; http://n2t.net/addgene:59187 ; RRID:Addgene_59187)
  • For your References section:

    CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme. Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM. GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063. 10.1080/21645698.2015.1106063 PubMed 26479970