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(Plasmid #59221)


Item Catalog # Description Quantity Price (USD)
Plasmid 59221 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pNDam-myc, Addgene plasmid 59217
  • Backbone manufacturer
    van Steensal lab
  • Total vector size (bp) 10271
  • Vector type
    Insect Expression ; DamID

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    Suppressor of variegation 205
  • Alt name
  • Alt name
    heterochromatin protein 1
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    Su(var)205 (a.k.a. Dmel_CG8409, CBX5, CG8409, DmHP-1, DmHP1, Dmel\CG8409, E(var)29, E(var)29A, FBgn0003607, HP-1, HP1, HP1-VS, HP1A, HP1a, HP1alpha, Hp1, Hp1a, SU(VAR)205, Su(Var)2-5, Su(Var)205, Su(var), Su(var)2-05, Su(var)2-5, Su(var)2-501, Su(var)29A, Su(var)[205], Su-var(2)5, Su[Var]205, Su[var]205, Sur(var)205, Suvar(2)5, Suvar2-5, dHP-1a, dHP1, dHP1a, hp1, hp1a, su(var)205)
  • Promoter Drosophila heat shock promoter
  • Tags / Fusion Proteins
    • Dam (N terminal on backbone)
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pCasper-hs
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDam-Myc-HP1 was a gift from Bas van Steensel (Addgene plasmid # 59221 ; ; RRID:Addgene_59221)
  • For your References section:

    Identification of in vivo DNA targets of chromatin proteins using tethered dam methyltransferase. van Steensel B, Henikoff S. Nat Biotechnol. 2000 Apr;18(4):424-8. 10.1038/74487 PubMed 10748524