pET21a(+) LdtMt2 (131-408)
(Plasmid
#59337)
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PurposeExpresses LdtMt2 for L,D-transpeptidation
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59337 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET21a(+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5443
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLdtMt2 (131-408)
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Alt nameMt2594
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Alt nameRv2518c
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SpeciesMycobacterium tuberculosis
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Insert Size (bp)834
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GenBank ID
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET21a(+) LdtMt2 (131-408) was a gift from SeWon Suh (Addgene plasmid # 59337 ; http://n2t.net/addgene:59337 ; RRID:Addgene_59337) -
For your References section:
Structural basis for the inhibition of Mycobacterium tuberculosis L,D-transpeptidase by meropenem, a drug effective against extensively drug-resistant strains. Kim HS, Kim J, Im HN, Yoon JY, An DR, Yoon HJ, Kim JY, Min HK, Kim SJ, Lee JY, Han BW, Suh SW. Acta Crystallogr D Biol Crystallogr. 2013 Mar;69(Pt 3):420-31. doi: 10.1107/S0907444912048998. Epub 2013 Feb 16. 10.1107/S0907444912048998 PubMed 23519417