pTE100
(Plasmid
#59395)
-
Purpose(Empty Backbone) MethyloBrick cloning vector, no promoter; TcR
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 59395 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepCM80
-
Backbone manufacturerLidstrom Lab
- Backbone size (bp) 7245
-
Modifications to backboneremoved promoter region; introduced new MCS
-
Vector typeBacterial Expression, Synthetic Biology
- Promoter none
Growth in Bacteria
-
Bacterial Resistance(s)Tetracycline, 10 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Brick_fw_Seq
- 3′ sequencing primer pCM80_rev (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The original backbone plasmid, pCM80, is published in Marx & Lidstrom, 2001, Microbiology: Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTE100 was a gift from Tobias Erb (Addgene plasmid # 59395 ; http://n2t.net/addgene:59395 ; RRID:Addgene_59395) -
For your References section:
A Set of Versatile Brick Vectors and Promoters for the Assembly, Expression, and Integration of Synthetic Operons in Methylobacterium extorquens AM1 and Other Alphaproteobacteria. Schada von Borzyskowski L, Remus-Emsermann M, Weishaupt R, Vorholt JA, Erb TJ. ACS Synth Biol. 2014 Aug 8. 10.1021/sb500221v PubMed 25105793
