RNAseq_cal_Tuschl
(Plasmid #59426)

• Purpose: Encodes sequence non-cognate to human genome for generation of RNAseq calibrators
• Depositing Lab: Thomas Tuschl
• Publication: Max et al Proc Natl Acad Sci U S A. 2018 Jun 5;115(23):E5334-E5343. doi: 10.1073/pnas.1714397115. Epub 2018 May 18.

Backbone

• Vector backbone: pMA-RQ-Bb
• Backbone manufacturer: Life Technologies
• Backbone size w/o insert (bp): 2341
• Total vector size (bp): 3549

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RNAseq_cal_Tuschl
• Insert Size (bp): 1208

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SfiI (not destroyed)
• 3′ cloning site: SfiI (not destroyed)
• 5′ sequencing primer: Custom made
• 3′ sequencing primer: Custom made

Resource Information

• Supplemental Documents:
  • RNAseq_cal_Tuschl gb file
• A portion of this plasmid was derived from a plasmid made by: Custom gene synthesis from Life Technologies

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See also PMID: 34850840 for an additional reference.