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RP185 pUAST EGFP:Or47b
(Plasmid #59525)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59525 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 8904
  • Total vector size (bp) 10604
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFP:Or47b
  • Alt name
    Or47b
  • Alt name
    Odorant receptor 47b
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2100
  • Entrez Gene
    Or47b (a.k.a. Dmel_CG13206, 47E.2, 47b, CG13206, DOR25, DOR47E.2, Dmel\CG13206, OR 47b, OR47b, Or47E.2)
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer Pry1 (5'-ccttagcatgtccgtggggtttgaat-3'); EGFP-C (5'-CATGGTCCTGCTGGAGTTCGTG-3')
  • 3′ sequencing primer SV40-splice-R2 (5'-CACAAACAATTAGAATCAGTAG-3')
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Note: Compared to the NCBI genomic reference sequence for Drosophila melanogaster Or47b , Addgene's quality control sequencing finds about ten mismatches in the insert ORF, including I245M and G363D amino acid residue substitutions. The depositing laboratory is aware of these differences, and states that they are polymorphisms that are not expected to affect protein function. The coding changes are not in highly conserved domains in the protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RP185 pUAST EGFP:Or47b was a gift from Leslie Vosshall (Addgene plasmid # 59525)
  • For your References section:

    Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. Benton R, Sachse S, Michnick SW, Vosshall LB. PLoS Biol. 2006 Feb;4(2):e20. Epub 2006 Jan 17. 10.1371/journal.pbio.0040020 PubMed 16402857