pSinRep5-181/25ic
(Plasmid #60078)

• Purpose: Chikungunya virus vaccine strain 181/25 infectious clone
• Depositing Lab: Terence Dermody
• Publication: Mainou et al MBio. 2013 Jul 2;4(4). pii: e00405-13. doi: 10.1128/mBio.00405-13.

Backbone

• Vector backbone: pSinRep5
• Backbone manufacturer: made by Charles McGee
• Backbone size w/o insert (bp): 1842
• Total vector size (bp): 13905
• Modifications to backbone: Sindbis virus coding sequences removed. Only sequences necessary for replication and selection in E. coli maintained.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Chikungunya virus strain 181/25 infectious clone
• Alt name: vaccine strain
• Alt name: 181/25
• Species: Chikungunya virus strain 181/25
• Insert Size (bp): 12063
• GenBank ID: L37661.3
• Promoter: SP6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: SP6 forward primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry