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Myopathy Loop Mutant Nuclear Myosin I YFP
(Plasmid #60243)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60243 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 60243-D50 50 μg of DNA in Tris buffer $495
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DNA 60243-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEYFP-N1
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7923
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nuclear Myosin 1
  • Alt name
    nuclear myosin 1B
  • Alt name
    Unconventional myosin-Ic
  • Species
    M. musculus (mouse)
  • Mutation
    Deleted I339, I340, G343, E344, E345,L346
  • GenBank ID
    NM_001080774.1
  • Entrez Gene
    Myo1c (a.k.a. MMIb, MYO1E, NMI, mm1beta, myr2)
  • Promoter CMV
  • Tags / Fusion Proteins
    • EYFP (C terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ECORI (not destroyed)
  • 3′ cloning site ECORI (not destroyed)
  • 5′ sequencing primer Unknown
  • 3′ sequencing primer Unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Myopathy Loop Mutant Nuclear Myosin I YFP was a gift from Primal de Lanerolle (Addgene plasmid # 60243 ; http://n2t.net/addgene:60243 ; RRID:Addgene_60243)

  • For your References section:

    Long-range directional movement of an interphase chromosome site. Chuang CH, Carpenter AE, Fuchsova B, Johnson T, de Lanerolle P, Belmont AS. Curr Biol. 2006 Apr 18;16(8):825-31. 10.1016/j.cub.2006.03.059 PubMed 16631592
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