|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60431||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeZFN donor
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Insert Size (bp)708
- Promoter CAG
- Cloning method Gibson Cloning
- 5′ sequencing primer aacaacgccaagtcatac
- 3′ sequencing primer ggagaagccttgctgaca (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAVS1-SA-2A-NEO-CAG-RTTA3 was a gift from Paul Gadue (Addgene plasmid # 60431 ; http://n2t.net/addgene:60431 ; RRID:Addgene_60431)
For your References section:A Doxycycline-Inducible System for Genetic Correction of iPSC Disease Models. Sim X, Cardenas-Diaz FL, French DL, Gadue P. Methods Mol Biol. 2015 Jan 29. 10.1007/7651_2014_179 PubMed 25630922