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pMSCV-Pidd1(T788A)
(Plasmid #60529)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60529 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMSCV
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 10000
  • Modifications to backbone
    JL090 MCS (HindIII-ApaI) cloned into pMSCV vector using HindIII and ApaI
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pidd1
  • Alt name
    Lrdd1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2800
  • Mutation
    Threonine 788 to Alanine*
  • GenBank ID
    NM_145886
  • Entrez Gene
    PIDD1 (a.k.a. LRDD, PIDD)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer TGACACGGTGGAGATGTTC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Antoine Tinel in Jurg Tschopp's lab cloned pMSCV-Pidd1. We generated point mutation using site-directed mutagenesis. *The Tschopp clone also contains V806D and R877H mutations, which are not known to affect function.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-Pidd1(T788A) was a gift from Trudy Oliver (Addgene plasmid # 60529 ; http://n2t.net/addgene:60529 ; RRID:Addgene_60529)
  • For your References section:

    Caspase-2 impacts lung tumorigenesis and chemotherapy response in vivo. Terry MR, Arya R, Mukhopadhyay A, Berrett KC, Clair PM, Witt B, Salama ME, Bhutkar A, Oliver TG. Cell Death Differ. 2014 Oct 10. doi: 10.1038/cdd.2014.159. 10.1038/cdd.2014.159 PubMed 25301067