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pDEST-hPDI
(Plasmid #60555)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60555 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST-0GWA
  • Backbone size (bp) 7105
  • Modifications to backbone
    insert His6-hPDI tag
  • Vector type
    Bacterial Expression
  • Tag / Fusion Protein
    • His6-hPDI (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    Must be grown in a strain resistant to ccdB
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-hPDI was a gift from Han Choe (Addgene plasmid # 60555 ; http://n2t.net/addgene:60555 ; RRID:Addgene_60555)
  • For your References section:

    Expression and purification of biologically active human FGF2 containing the b'a' domains of human PDI in Escherichia coli. Song JA, Koo BK, Chong SH, Kwak J, Ryu HB, Nguyen MT, Vu TT, Jeong B, Kim SW, Choe H. Appl Biochem Biotechnol. 2013 May;170(1):67-80. doi: 10.1007/s12010-013-0140-3. Epub 2013 Mar 8. 10.1007/s12010-013-0140-3 PubMed 23471584