pHL-H1-ccdB-mEF1a-RiH
(Plasmid #60601)

• Purpose: Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.
• Depositing Lab: Akitsu Hotta
• Publication: Li et al Stem Cell Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 10.1016/j.stemcr.2014.10.013.

Backbone

• Vector backbone: pHL-MCS1
• Backbone manufacturer: Hotta Lab, CiRA
• Backbone size w/o insert (bp): 2043
• Total vector size (bp): 6946
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Growth instructions: ccdB resistant strain of E.coli is required.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Red Fluorescent Protein
• Alt name: mRFP1
• Species: Discosoma sp
• Insert Size (bp): 678

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: BlnI (not destroyed)
• 5′ sequencing primer: N.A.
• 3′ sequencing primer: N.A.

Gene/Insert 2

• Gene/Insert name: Hygromycin resistance gene
• Alt name: HygroR
• Species: E.coli
• Insert Size (bp): 1026

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: N.A.
• 3′ sequencing primer: N.A.

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry