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(Plasmid #60901)


Item Catalog # Description Quantity Price (USD)
Plasmid 60901 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pUC ori vector
  • Backbone size (bp) 9070
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter CBh and U6
  • Tag / Fusion Protein
    • 3XFLAG (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The plasmid is generated by replacing Cas9 D10A nickase in pX335 from the Zhang laboratory with FokI-dCas9 from pSQT1601 from the Joung laboratory.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The gRNA cloning strategy is exactly the same as for pX330/pX335.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXFokI-dCas9 was a gift from Bruce Conklin (Addgene plasmid # 60901 ; ; RRID:Addgene_60901)
  • For your References section:

    Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing. Miyaoka Y, Berman JR, Cooper SB, Mayerl SJ, Chan AH, Zhang B, Karlin-Neumann GA, Conklin BR. Sci Rep. 2016 Mar 31;6:23549. doi: 10.1038/srep23549. 10.1038/srep23549 PubMed 27030102