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(Plasmid #60999)


Item Catalog # Description Quantity Price (USD)
Plasmid 60999 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 2700
  • Total vector size (bp) 3790
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    TTE1564 transcript
  • Species
    Thermoanaerobacter tengcongensis MB4
  • Insert Size (bp)
  • GenBank ID
    995666 995665
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13 Fwd
  • 3′ sequencing primer tgtggaattgtgagcgga
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19_extendedTTE1564 was a gift from Nils Walter (Addgene plasmid # 60999 ; ; RRID:Addgene_60999)
  • For your References section:

    The Shine-Dalgarno sequence of riboswitch-regulated single mRNAs shows ligand-dependent accessibility bursts. Rinaldi AJ, Lund PE, Blanco MR, Walter NG. Nat Commun. 2016 Jan 19;7:8976. doi: 10.1038/ncomms9976. 10.1038/ncomms9976 PubMed 26781350