pAMB_CAT-FspI
(Plasmid
#61002)
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PurposeT7-transcription template for CAT control mRNA used for in vitro translation
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61002 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAMB
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Backbone manufacturerAmbion
- Total vector size (bp) 3210
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)JM109
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameChloramphenicol acetyltransferase
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Insert Size (bp)657
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MutationFspI site introduced after T7 terminator
- Promoter T7
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer TCTGACACATGCAGCTCC (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMutagenesis was done on control vector provided with the ActivePro™ In Vitro Translation Kit (Ambion)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAMB_CAT-FspI was a gift from Nils Walter (Addgene plasmid # 61002 ; http://n2t.net/addgene:61002 ; RRID:Addgene_61002) -
For your References section:
The Shine-Dalgarno sequence of riboswitch-regulated single mRNAs shows ligand-dependent accessibility bursts. Rinaldi AJ, Lund PE, Blanco MR, Walter NG. Nat Commun. 2016 Jan 19;7:8976. doi: 10.1038/ncomms9976. 10.1038/ncomms9976 PubMed 26781350
Map uploaded by the depositor.
