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pJWV25
(Plasmid #61045)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61045 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPPP1
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Tag / Fusion Protein
    • GFP+ (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Ampicillin selection in E. coli, tetracycline selection in S. pneumoniae.
  • Copy number
    High Copy

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJWV25 was a gift from Jan-Willem Veening (Addgene plasmid # 61045 ; http://n2t.net/addgene:61045 ; RRID:Addgene_61045)
  • For your References section:

    Cellular localization of choline-utilization proteins in Streptococcus pneumoniae using novel fluorescent reporter systems. Eberhardt A, Wu LJ, Errington J, Vollmer W, Veening JW. Mol Microbiol. 2009 Oct;74(2):395-408. doi: 10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8. 10.1111/j.1365-2958.2009.06872.x PubMed 19737355