pmCherry_C1 I3
(Plasmid #61398)

• Purpose: Expresses mCherry-tagged Inhibitor-3 in mammalian cells
• Depositing Lab: Hemmo Meyer
• Publication: Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Backbone

• Vector backbone: pEGFP_C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5100
• Modifications to backbone: GFP was exchanged by mCherry via restriction digest with AgeI and EcoRI
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Inhibitor-3
• Alt name: PPP1R11
• Species: H. sapiens (human)
• Entrez Gene: PPP1R11 (a.k.a. CFAP255, HCG-V, HCGV, IPP3, TCTE5, TCTEX5)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: pEGFP-RP

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry