PurposeExpresses mCherry tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61399||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5100
Modifications to backboneGFP was exchanged by mCherry via restriction digest with AgeI and EcoRI
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Mutationsilent mutations of bases C183T T192C T189C
Entrez GenePPP1R11 (a.k.a. CFAP255, HCG-V, HCGV, IPP3, TCTE5, TCTEX5)
- Promoter CMV
/ Fusion Protein
- mCherry (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer pEGFP-RP (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
resistant against siRNA oligomer I3 S2 (CUGCUGTAUUUAUGAGAAA)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pmCherry_C1 I3(resist) was a gift from Hemmo Meyer (Addgene plasmid # 61399 ; http://n2t.net/addgene:61399 ; RRID:Addgene_61399)
For your References section:Inhibitor-3 ensures bipolar mitotic spindle attachment by limiting association of SDS22 with kinetochore-bound protein phosphatase-1. Eiteneuer A, Seiler J, Weith M, Beullens M, Lesage B, Krenn V, Musacchio A, Bollen M, Meyer H. EMBO J. 2014 Oct 8. pii: e201489054. 10.15252/embj.201489054 PubMed 25298395