miR-144-451 Trap
(Plasmid
#61488)
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Purposeto knockdown endogenous miR144 and miR451 levels
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61488 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonebiPGK
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Backbone manufacturerNaldinin lab, Brown, et al. 2007 PMID 18026085
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemiR-144-451 Trap
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Alt namemiR 144 Trap inserted downstream of miR-451 trap
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Insert Size (bp)530
- Promoter bidirectional phosphoglycerate kinase promoter (biPGK)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XmaI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer biPGK-F (CTGACAATTCCGTGGTGTTGTCGC)
- 3′ sequencing primer biPGK-R (CCCAGGCTCAGATCTGGTCTAACC) (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
miR-144-451 Trap was a gift from Curt Civin (Addgene plasmid # 61488 ; http://n2t.net/addgene:61488 ; RRID:Addgene_61488) -
For your References section:
MIR144 and MIR451 regulate human erythropoiesis via RAB14. Kim M, Tan YS, Cheng WC, Kingsbury TJ, Heimfeld S, Civin CI. Br J Haematol. 2014 Oct 14. doi: 10.1111/bjh.13164. 10.1111/bjh.13164 PubMed 25312678