PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TA mutation
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61513||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Gene/Insert nameFK506 binding protein 1A
SpeciesH. sapiens (human)
Entrez GeneFKBP1A (a.k.a. FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE)
- Promoter CMV
/ Fusion Proteins
- mCherry (N terminal on insert)
- Tom20 (N terminal on insert)
- AIP with TA mutation (see comments) (C terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer SV40 poly A Reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
T429A in AIP following (in-frame with) FKBP caused by GA mismatch at 1313 bp and TC mismatch at 1315 bp in Author's partial sequence (please refer to Tom20-mChF-AIP for comparison).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Tom20-mChF-AIP(TA) was a gift from Takanari Inoue (Addgene plasmid # 61513 ; http://n2t.net/addgene:61513 ; RRID:Addgene_61513)
For your References section:Compartmentalized AMPK signaling illuminated by genetically encoded molecular sensors and actuators. Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, Chen M, Tokumitsu H, Zhang J, Inoue T. Cell Rep. 2015 Apr 28;11(4):657-70. doi: 10.1016/j.celrep.2015.03.057. Epub 2015 Apr 16. 10.1016/j.celrep.2015.03.057 PubMed 25892241