pOPINK-TRABID (OTU+AnkUBD, aa 245-697)
(Plasmid #61590)

• Purpose: Expresses human TRABID (OTU+AnkUBD domains) in E. coli.
• Depositing Lab: David Komander
• Publication: Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):62-71. doi: 10.1038/nsmb.2169.

Backbone

• Vector backbone: pOPINK
• Backbone manufacturer: OPPF-UK
• Backbone size w/o insert (bp): 6299
• Total vector size (bp): 7329
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TRABID
• Alt name: Ubiquitin thioesterase ZRANB1
• Species: H. sapiens (human)
• Insert Size (bp): 1359
• Mutation: Deleted aa 1-244 and aa 698-708.
• GenBank ID: NM_017580.2
• Entrez Gene: ZRANB1 (a.k.a. TRABID)
• Promoter: T7
• Tag / Fusion Protein:
  • His6-GST-3C (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (destroyed during cloning)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pGEX 5’
• 3′ sequencing primer: T7 Terminal

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046.

Hospenthal MK, Mevissen TET, Komander D. Nature Protoc. 2015 Jan 29;10:349-361. doi:10.1038/nprot.2015.018