SNAP-EF(N)-30nmSAH-GFP(1-10)
(Plasmid
#61662)
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Purpose30nm SAH scaffold with EF Hand and split GFP attachment sites
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Depositing Lab
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Depositing OrganizationUniversity of Michigan
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61662 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 61662-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 61662-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneC1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4013
- Total vector size (bp) 6084
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSNAP-EF(N)-30nmSAH-GFP(1-10)
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Insert Size (bp)2148
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GFP(1-10) sequence provided by F Pinaud [(2011) PNAS 108:E301-E210] based on work in the lab of G Waldo engineering the split GFP to function as a self assembling bipartite protein [Cabantous et al. (2005) Nat Biotechnol 23:102–107].
DNA (Catalog # 61662-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 61662-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SNAP-EF(N)-30nmSAH-GFP(1-10) was a gift from Kristen Verhey (Addgene plasmid # 61662 ; http://n2t.net/addgene:61662 ; RRID:Addgene_61662) -
For your References section:
A method for multiprotein assembly in cells reveals independent action of kinesins in complex. Norris SR, Soppina V, Dizaji AS, Schimert KI, Sept D, Cai D, Sivaramakrishnan S, Verhey KJ. J Cell Biol. 2014 Nov 10;207(3):393-406. doi: 10.1083/jcb.201407086. Epub 2014 Nov 3. 10.1083/jcb.201407086 PubMed 25365993
Map uploaded by the depositor.
