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Purpose(Empty Backbone) Gateway compatible Y2H vector - AD
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61702 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGADT7
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Backbone manufacturerClontech
- Backbone size (bp) 7988
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Modifications to backboneGateway cassette was cloned into the MCS
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGADT7-GW was a gift from Yuhai Cui (Addgene plasmid # 61702 ; http://n2t.net/addgene:61702 ; RRID:Addgene_61702) -
For your References section:
Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y. Plant J. 2010 Jan;61(2):259-70. doi: 10.1111/j.1365-313X.2009.04048.x. Epub 2009 Oct 16. 10.1111/j.1365-313X.2009.04048.x PubMed 19843313
Map uploaded by the depositor.
