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Addgene

p414CYC_Hsp82_noTER
(Plasmid #61716)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61716 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p414
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    6xHis-Hsp82
  • Alt name
    Hsp90
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2000
  • Entrez Gene
    HSP82 (a.k.a. YPL240C, HSP90)
  • Promoter CYC1
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cagctatgaccatgattacg
  • 3′ sequencing primer gtaaaacgacggccagtgagc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p414CYC_Hsp82_noTER was a gift from Dan Bolon (Addgene plasmid # 61716 ; http://n2t.net/addgene:61716 ; RRID:Addgene_61716)

  • For your References section:

    Latent effects of Hsp90 mutants revealed at reduced expression levels. Jiang L, Mishra P, Hietpas RT, Zeldovich KB, Bolon DN. PLoS Genet. 2013 Jun;9(6):e1003600. doi: 10.1371/journal.pgen.1003600. Epub 2013 Jun 27. PGENETICS-D-12-02384 [pii] PubMed 23825969
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