pCDNA3 NQO1-Flag
(Plasmid #61729)

• Purpose: Expresses human NQO1 Flag tagged at C-terminus
• Depositing Lab: Yosef Shaul
• Publication: Tsvetkov et al J Biol Chem. 2011 Mar 18;286(11):8839-45. doi: 10.1074/jbc.M110.193276. Epub 2011 Jan 10.

Backbone

• Vector backbone: pCDNA3
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6225
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NAD(P)H dehydrogenase, quinone 1 (NQO1)
• Species: H. sapiens (human)
• Insert Size (bp): 825
• GenBank ID: NM_000903.2
• Entrez Gene: NQO1 (a.k.a. DHQU, DIA4, DTD, NMOR1, NMORI, QR1)
• Promoter: cmv
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (not destroyed)
• 3′ cloning site: BamHI-vector, BglII-insert (destroyed during cloning)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry