pcDNA3.1(+) neo with Smad5 CDS
(Plasmid
#61800)
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PurposepcDNA3.1(+) with full length mouse Smad5 CDS
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61800 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 6000
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmad5 CDS
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SpeciesM. musculus (mouse)
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Insert Size (bp)1397
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Entrez GeneSmad5 (a.k.a. 1110051M15Rik, Dwf-C, Madh5, MusMLP)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+) neo with Smad5 CDS was a gift from Judy Lieberman (Addgene plasmid # 61800 ; http://n2t.net/addgene:61800 ; RRID:Addgene_61800) -
For your References section:
miR-200 promotes the mesenchymal to epithelial transition by suppressing multiple members of the Zeb2 and Snail1 transcriptional repressor complexes. Perdigao-Henriques R, Petrocca F, Altschuler G, Thomas MP, Le MT, Tan SM, Hide W, Lieberman J. Oncogene. 2015 Mar 23. doi: 10.1038/onc.2015.69. 10.1038/onc.2015.69 PubMed 25798844