pT7 cyclin A1-164 venus cks1 (2196)
(Plasmid #61839)

• Purpose: expression of N terminal cyclin A and cks1
• Depositing Lab: Jonathon Pines
• Publication: Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

Backbone

• Vector backbone: pt7 venus cks1 (1843)
• Backbone manufacturer: Pines Lab
• Vector type: in vitro transcription

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: cyclin A
• Alt name: csk1
• Species: H. sapiens (human)
• Mutation: L2V
• Entrez Gene: CCNA2 (a.k.a. CCN1, CCNA)
• Promoter: T7
• Tag / Fusion Protein:
  • Venus (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry