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pDEST15.1-hRPN13 (285-407) (Q337A/Q338A)
(Plasmid #61935)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 61935 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST15
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 7400
  • Modifications to backbone
    GSFT sequence after TEV site mutated to GSWT to aid in quantification by E280.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hRPN13 (285-407)
  • Alt name
    ADRM1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    400
  • Mutation
    Construct starts at residue 285, residues Q337 and Q338 mutated to Alanines
  • GenBank ID
    NM_175573
  • Entrez Gene
    ADRM1 (a.k.a. ARM-1, ARM1, GP110)
  • Promoter T7
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Q337A/Q338A mutations incorporated via Quickchange mutagenesis

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST15.1-hRPN13 (285-407) (Q337A/Q338A) was a gift from Chris Hill (Addgene plasmid # 61935 ; http://n2t.net/addgene:61935 ; RRID:Addgene_61935)
  • For your References section:

    Structural Basis for the Activation and Inhibition of the UCH37 Deubiquitylase. VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson H, Cohen RE, Yao T, Hill CP. Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 10.1016/j.molcel.2015.01.016. 10.1016/j.molcel.2015.01.016 PubMed 25702872