pSABAD14-172
(Plasmid
#61976)
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PurposePhoRadAC6 intein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61976 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 4597
- Total vector size (bp) 4822
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePhoRadAC6 intein
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Alt namePhoRadAC6 C-intein
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SpeciesPyrococcus horikoshii
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Insert Size (bp)225
- Promoter pBAD
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Tag
/ Fusion Protein
- GB1-H6 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer pBAD Forward
- 3′ sequencing primer pBAD Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSABAD14-172 was a gift from Hideo Iwai (Addgene plasmid # 61976 ; http://n2t.net/addgene:61976 ; RRID:Addgene_61976) -
For your References section:
Structure-based engineering and comparison of novel split inteins for protein ligation. Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H. Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h. 10.1039/c4mb00021h PubMed 24574026