NKP33 GFP (1032)
Purposemammalian expression of nuclear envelope transmembrane protein
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||62022||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GenePLGRKT (a.k.a. AD025, C9orf46, MDS030, PLG-RKT, Plg-R(KT))
- Promoter CMV
/ Fusion Protein
- EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:NKP33 GFP (1032) was a gift from Eric Schirmer (Addgene plasmid # 62022 ; http://n2t.net/addgene:62022 ; RRID:Addgene_62022)
For your References section:The nuclear envelope proteome differs notably between tissues. Korfali N, Wilkie GS, Swanson SK, Srsen V, de Las Heras J, Batrakou DG, Malik P, Zuleger N, Kerr AR, Florens L, Schirmer EC. Nucleus. 2012 Nov-Dec;3(6):552-64. doi: 10.4161/nucl.22257. Epub 2012 Sep 18. 10.4161/nucl.22257 PubMed 22990521