BiP-mGFP P495L
(Plasmid #62232)

• Purpose: Mutant of hamster BiP-mGFP unable to bind clients.
• Depositing Lab: Erik Snapp
• Depositing Organization: Albert Einstein College of Medicine
  Located in United States of America
• Publication: Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-21. doi: 10.1091/mbc.E09-12-1066. Epub 2010 Apr 21.

Backbone

• Vector backbone: clontech N1
• Backbone manufacturer: clontech
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 6700
• Vector type: Mammalian Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hamster BiP-mGFP-KDEL
• Species: Synthetic; hamster
• Insert Size (bp): 2702
• Promoter: cmv
• Tag / Fusion Protein:
  • mGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: notI (not destroyed)
• 5′ sequencing primer: tggtttagtgaaccgt
• 3′ sequencing primer: GTTCAGGGGGAGGTGTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Avoid overexpression. Low expressing cells are best for imaging. High expression leads to protein aggregation in the nuclear envelope. See supplementary data for original paper.
This mutant can not bind client proteins.
Do NOT use this as an ER marker. BiP is a functional protein and significantly impacts the ER environment increasing stress resistance. Use ER-GFP if you simply want a marker for the ER when imaging cells.

DNA (Catalog # 62232-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 62232-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.