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Proinsulin-NanoLuc in pLX304
(Plasmid #62057)


Item Catalog # Description Quantity Price (USD)
Plasmid 62057 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 9377
  • Total vector size (bp) 8574
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    M. musculus (mouse), Synthetic
  • Insert Size (bp)
  • Mutation
    Inserted NanoLuc into C-peptide of mouse Ins2 coding sequence, with added PC2 sites flanking the luciferase
  • Entrez Gene
    Ins2 (a.k.a. Ins-2, InsII, Mody, Mody4)
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Proinsulin-NanoLuc in pLX304 was a gift from David Altshuler (Addgene plasmid # 62057 ; ; RRID:Addgene_62057)
  • For your References section:

    High-throughput luminescent reporter of insulin secretion for discovering regulators of pancreatic Beta-cell function. Burns SM, Vetere A, Walpita D, Dancik V, Khodier C, Perez J, Clemons PA, Wagner BK, Altshuler D. Cell Metab. 2015 Jan 6;21(1):126-37. doi: 10.1016/j.cmet.2014.12.010. 10.1016/j.cmet.2014.12.010 PubMed 25565210