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Addgene

Proinsulin-NanoLuc in pLX304 Notes

Addgene Diagnostic Test

This gel depicts the NEB 1kb ladder and a digestion of the plasmid 62057 with the following enzymes: XhoI and NheI-HF.

We expect the linearized plasmid to give 1 band of 8.5kb and the double digest to give 2 bands of ~7kb and 1.5kb. We see the expected pattern, although the 1.5kb band is very faint.