H6-auxilin
(Plasmid #62571)

• Purpose: 6xHis-tagged full-length bovine brain auxilin (auxilin 1) in pQE30 for protein expression in bacteria
• Depositing Lab: Ernst Ungewickell
• Publication: Schroder et al Eur J Biochem. 1995 Mar 1;228(2):297-304.

Backbone

• Vector backbone: pQE30
• Backbone manufacturer: Qiagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: The depositing laboratory recommends strain BL21 for protein expression.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Auxilin
• Species: B. taurus (bovine)
• Insert Size (bp): 3000
• Mutation: *see comment below
• Tag / Fusion Protein:
  • 6XHis (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: PstI (not destroyed)
• 5′ sequencing primer: pBRforEco (5'-AATAGGCGTATCACGAGGC-3')
• 3′ sequencing primer: T0term-R (5'-GCGGCAACCGAGCGTTC-3)'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*Note: Addgene's quality control sequencing finds a T234I amino acid residue substitution in the bovine auxilin gene. This residue change is not thought to affect protein function.