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pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)
(Plasmid #62577)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62577 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 62577-D50 50 μg of DNA in Tris buffer $495
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DNA 62577-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pMIR-Report
  • Backbone manufacturer
    Ambion
  • Backbone size w/o insert (bp) 6470
  • Total vector size (bp) 7700
  • Modifications to backbone
    To construct a WT SPRED1 reporter containing the putative miR-206 binding site, the 1.2-kb SPRED1 3′ UTR from clone BG167687 was excised by treatment with MluI and inserted into the same site of pMIR-REPORT.
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SPRED1
  • Alt name
    Sprouty Related EVH1 domain containing 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Entrez Gene
    SPRED1 (a.k.a. LGSS, NFLS, PPP1R147, hSpred1, spred-1)

Cloning Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIR-Report-SPRED1-3'UTR (WT - miR-206 site) was a gift from Michael Ruppert (Addgene plasmid # 62577 ; http://n2t.net/addgene:62577 ; RRID:Addgene_62577)

  • For your References section:

    MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1. Sharma SB, Lin CC, Farrugia MK, McLaughlin SL, Ellis EJ, Brundage KM, Salkeni MA, Ruppert JM. Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 10.1128/MCB.00480-14. Epub 2014 Sep 8. 10.1128/MCB.00480-14 PubMed 25202123
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