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(Plasmid #62593)


Item Catalog # Description Quantity Price (USD)
Plasmid 62593 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size (bp) 6840
  • Modifications to backbone
    LacZ screening cassette flanked by BbsI sites for golden gate cloning
  • Vector type
    Mammalian Expression, TALEN ; T7
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3× FLAG (N terminal on backbone)
    • FOKI WT (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This material was constructed from JDS78 (AddGene Plasmid #32290). The TALEN insertion site was replaced with a LacZ selection cassette flanked by two BbsI sites. Other BbsI sites in the plasmid backbone were mutated.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AI-CN443 was a gift from Joshua Grimley (Addgene plasmid # 62593 ; ; RRID:Addgene_62593)
  • For your References section:

    Genome engineering of isogenic human ES cells to model autism disorders. Martinez RA, Stein JL, Krostag AR, Nelson AM, Marken JS, Menon V, May RC, Yao Z, Kaykas A, Geschwind DH, Grimley JS. Nucleic Acids Res. 2015 Mar 11. pii: gkv164. 10.1093/nar/gkv164 PubMed 25765640