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PurposeRetrovirus expression plasmid
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62924 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBABE-puro
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Backbone manufacturerAddgene plasmid # 1764
- Backbone size w/o insert (bp) 5169
- Total vector size (bp) 6454
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIDH1
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Alt nameIsocitrate Dehydrogenase
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SpeciesH. sapiens (human)
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Mutationmutation Arg132His
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GenBank IDNM_001282386.1
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Entrez GeneIDH1 (a.k.a. HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD)
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe-puro-Flag-IDH1-R132H was a gift from Yue Xiong (Addgene plasmid # 62924 ; http://n2t.net/addgene:62924 ; RRID:Addgene_62924) -
For your References section:
Glioma-derived mutations in IDH1 dominantly inhibit IDH1 catalytic activity and induce HIF-1alpha. Zhao S, Lin Y, Xu W, Jiang W, Zha Z, Wang P, Yu W, Li Z, Gong L, Peng Y, Ding J, Lei Q, Guan KL, Xiong Y. Science. 2009 Apr 10;324(5924):261-5. doi: 10.1126/science.1170944. 10.1126/science.1170944 PubMed 19359588
Map uploaded by the depositor.
