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AAV-FLIM-AKAR
(Plasmid #63058)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63058 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV-ChR2-mCherry
  • Backbone manufacturer
    Arpiar Saunders and Bernardo Sabatini
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7300
  • Modifications to backbone
    N/A
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use recombinase-free E. coli (e.g. Invitrogen's Stbl3)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLIM-AKAR
  • Species
    Synthetic
  • Insert Size (bp)
    1900
  • Promoter EF-1α

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TTCTTCCATTTCAGGTGTCG
  • 3′ sequencing primer GAGGTTGATTATCGATAAGC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-FLIM-AKAR was a gift from Bernardo Sabatini (Addgene plasmid # 63058 ; http://n2t.net/addgene:63058 ; RRID:Addgene_63058)
  • For your References section:

    A PKA activity sensor for quantitative analysis of endogenous GPCR signaling via 2-photon FRET-FLIM imaging. Chen Y, Saulnier JL, Yellen G, Sabatini BL. Front Pharmacol. 2014 Apr 2;5:56. doi: 10.3389/fphar.2014.00056. eCollection 2014. 10.3389/fphar.2014.00056 PubMed 24765076