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Addgene

pKG96
(Plasmid #63072)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 63072 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIC242
  • Backbone manufacturer
    Cheeseman/Desai (Addgene plasmid #44432)
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CENP-A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    423
  • Mutation
    A12V, C75Y, and L121F mutations compared to GenBank reference sequence NP_001800.1
  • Entrez Gene
    CENPA (a.k.a. CENP-A, CenH3)
  • Tags / Fusion Proteins
    • EGFP (N terminal on backbone)
    • TEV-S (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer oCB21
  • 3′ sequencing primer oCB22
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKG96 was a gift from Iain Cheeseman (Addgene plasmid # 63072 ; http://n2t.net/addgene:63072 ; RRID:Addgene_63072)
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