Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pKG96
(Plasmid #63072)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 63072 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIC242
  • Backbone manufacturer
    Cheeseman/Desai (Addgene plasmid #44432)
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CENP-A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    423
  • Mutation
    A12V, C75Y, and L121F mutations compared to GenBank reference sequence NP_001800.1
  • Entrez Gene
    CENPA (a.k.a. CENP-A, CenH3)
  • Tags / Fusion Proteins
    • EGFP (N terminal on backbone)
    • TEV-S (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer oCB21
  • 3′ sequencing primer oCB22
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKG96 was a gift from Iain Cheeseman (Addgene plasmid # 63072 ; http://n2t.net/addgene:63072 ; RRID:Addgene_63072)