pAAV CTG 700x
(Plasmid #63087)

• Purpose: AdenoAssociated Virus plasmid containing expanded repeats--CTG 700x that is the mutation found in Myotonic Dystrophic type 1
• Depositing Lab: Nicolas Charlet-Berguerand
• Publication: Charlet-Berguerand plasmids (unpublished)

Backbone

• Vector backbone: unknown
• Total vector size (bp): 7553
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CTG REPEATS
• Species: Synthetic
• Mutation: CTG 700x that is the mutation found in Myotonic Dystrophic type 1
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Supplemental Documents:
  • pAAV CMV CUG 700x annotated genbank file
• Addgene Notes:
  • Addgene Diagnostic Test
  • Addgene Diagnostic Test
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene cannot verify or guarantee the presence of all 700 CTG repeats in this array

Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.

This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.