pAAV CTG 700x
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||63087||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 7553
Vector typeMammalian Expression, AAV
Growth in Bacteria
Bacterial Resistance(s)low Amp
Growth TemperatureRoom Temperature
Growth Strain(s)NEB Stable
Gene/Insert nameCTG REPEATS
MutationCTG 700x that is the mutation found in Myotonic Dystrophic type 1
- Promoter CMV
/ Fusion Protein
- EGFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Addgene cannot verify or guarantee the presence of all 700 CTG repeats in this array
Should be amplified in STBL3 bacteria and grown at Room temperature to keep the repeats stable.
This plasmid should be verified via restriction analysis. Multiple colonies may need to be screened to isolate the correct construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV CTG 700x was a gift from Nicolas Charlet-Berguerand (Addgene plasmid # 63087 ; http://n2t.net/addgene:63087 ; RRID:Addgene_63087)